LSM 800
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Sample: courtesy of A. Seitz, EPFL, Lausanne, Switzerland

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Convallaria, rare event detection with ZEN OAD.

Perfectly Tailored to Your Needs

  • Use up to three highly sensitive GaAsP detectors
  • Perform fast linear scanning Profit from high productivity and throughput
  • Benefit from great flexibility in live cell imaging
  • Get uncompromised image quality
  • Perform precise quantitative measurements
  • Use Airyscan for 1.7 times higher resolution and higher sensitivity than any classic confocal

Open Interfaces to Extend Your System

  • Get the full benefits of integrated incubation solutions and state-of-the-art Axiocams for your lab or multi-user facility
  • Intuitive ZEN imaging software for complex automated imaging routines with Experiment Designer
  • Easily exchange data with third party software
  • Define your own application world using the powerful open application development (OAD)
  • Use ZEISS Shuttle & Find for correlative microscopy and connect LSM 800 with your ZEISS electron microscope
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Your Compact System for High-end Confocal Imaging

 LSM 800 makes excellent economic sense:

  • Get an affordable system with an attractive price / performance ratio that is robust and easy to use
  • Save lab space and money: LSM 800 has a small footprint and minimal setup requirements, minimal maintenance and training, is self-calibrating and has low energy consumption
  • Enjoy predictable cost of ownership over the entire lifetime

Plant root (Arabidopsis thaliana);
Sample: courtesy of T. Pasternak, Institute of Biology, University Freiburg, Germany

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Revolutionize Your Confocal Imaging with ZEISS Airyscan

Airyscan is an array detector that draws on the fact that a fluorescence microscope will image a point-like source as an extended airy disk. When you close the pinhole in a standard confocal microscope to reject out-of-focus light, you get a sharper image, but it’s also dimmer since a great deal of light is then lost. The smaller the pinhole, the higher the resolution, but – equally – the bigger the loss in light.

Airyscan solves this conundrum between resolution and light efficiency by imaging the complete airy disk onto a concentrically arranged hexagonal detector array. It consists of 32 single detector elements, all of which act like sub airy unit pinholes. The confocal pinhole itself remains open and doesn’t block light – thus all photons of the whole airy disk are collected.

The signals from all detector elements are then reassigned to their correct position, producing an image with increased signal-to-noise ratio and resolution. Unlike other superresolution techniques, Airyscan capitalizes on the scanning and optical sectioning capabilities of a confocal. Thus Airyscan even works with thicker samples such as tissue sections or whole animal mounts that need a higher penetration depth.

Airyscan detector, schematic beam path

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Isolated centrioles of Chlamydia;
Sample: courtesy of P. Guichard, EPFL, Lausanne, Switzerland

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