Zen Features

ZEN: Module Multi Channel


ZEN: Module Z Stack


ZEN Module Extended Focus


ZEN: Module Panorama


ZEN Tiles & Positions

With the ZEN Multi Channel module you create images with multiple channels. To do this, with widefield systems you combine any number of  fluorescence channels with images in transmitted light, e.g. phase  contrast, according to your requirements. A special feature here is that you can select your dyes from the integrated database. This already  contains data sets for more than 500 fluorochromes and can be expanded as required.

For each fluorescence excitation wavelength, the relevant channel is acquired with the optimal exposure time. An independent exposure time and microscope setting can also be  assigned to each channel. The advantage: you structure your experiments  flexibly and perform even highly complex multichannel experiments  simply.

The ZEN Z Stack module helps you to acquire axial image stacks on your ZEISS microscope with motorized focus drive. The program controls the Z-drive of your  stand in precisely the right increments and synchronizes it with  acquisition. ZEN can calculate the increments fully automatically,  if required, or you can allocate them manually.

A special feature here is that from now on you create your Z Stacks with graphical support. This assistance is even available to you dynamically during  acquisition. The size of your experiment, which is saved in a compact  .CZI file, is limited only by the travel range of your system and the  minimum increment.

With the Extended Focus module you generate images with extended depth  of field from previously acquired Z-stacks. The ZEN module extracts sharply imaged details from existing images at different focus  positions and combines them to create a new image. The advantage: you  always have maximum depth of field in all regions of your images in transmitted light, reflected light and fluorescence contrast.

This ZEN module is ideal if your specimens no longer fit into the image field. With Panorama you create high-resolution overview images from individual images. The software puts the images together with pixel precision. The advantage: even images that have been acquired offset from each other can be joined precisely using ZEN and Panorama. This means that important details from the individual images of your microscope specimen are recorded in a single image.

A special feature here is that you can use the live image as a ‘joystick’ and therefore acquire the individual images even more quickly.

With the optional ZEN Tiles & Positions module and a motorized stage you scan your specimen automatically, making it possible to analyze large areas. A large, virtual overview image can be generated in this way from numerous individual images. This allows you to navigate over the specimen like a map and zoom easily into any regions of interest. Tiles & Positions and ZEN align the individual images precisely in relation to one another, providing the optimal basis for further analyses

A special feature here is that a software wizard helps you to mark positions on multiwell plates, multi-chamber slides, slides and dishes interactively and locate them with a click of your mouse.

Lukas Microscope Service, Inc.            25715 N. Hillview Court, Mundelein, IL. 60060            847-673-2600

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