Optical sectioning using structured illumination


Optical Sections in Fluorescence Imaging

Create optical sections of your fluorescent samples – free of scattered  light. With structured illumination, you know that only the focal plane  appears in your image: ApoTome.2 recognizes the magnification and moves  the appropriate grid into the beampath. The system then calculates your  optical section from three images with different grid positions without  time lag. It’s a totally reliable way to prevent scattered out-of-focus  light, even in your thicker specimens. Operate your system just as easy  as always. You get images with high contrast in the best possible  resolution – simply brilliant optical sections.

Perfect Images – with All Magnifications

Because your applications need different objectives, you need a  system that gives you the best resolution for each one. ApoTome.2  automatically uses the right grid for your objective, selecting from  three grids with different frequencies. With a defined optical section  thickness in the region of a Rayleigh unit, you prpfit from simply  brilliant images.

Optimum Results – Free Choice of Light Source and Dyes

From conventional HBO illumination to adjustmentfree metal-halide  lamp HXP 120 C to Colibri.2, the LED illumination source that is gentle  on your samples: with ApoTome.2 you use exactly the light you need.  ApoTome.2 also gives you the choice of fluorophores. Whether you work  with DAPI, FITC, Rhodamin, Cy5 or with vital dyes such as GFP or mRFP,  it’s your decision, not the technology’s. Just change the filter and  your system automatically moves the grid to the correct position. From  DAPI to Cy5, you get perfect optical sections for multichannel imaging

Brilliant Images – Even with Thick Specimens

Your optical section thickness is close to one Rayleigh unit, a value that stands for high axial resolution with a good signal-to-noise  ratio. ApoTome.2 increases the resolution in Z direction compared to  conventional fluorescence microscopy: you obtain brilliant optical  sections that allow 3D-rendering , even from thick specimens.

Lukas Microscope Service, Inc.            25715 N. Hillview Court, Mundelein, IL. 60060            847-673-2600

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