Perform Quantitative Imaging

• Perform gentle imaging of your samples, thanks to the homogeneous illumination with linear scanning, combined with a sensitive detection infrastructure
• Working with identical pixel times and continuous scanner monitoring, you perform quantitative imaging at all speeds and scan modes
• Get robust and reliable results, even from your most demanding single molecule imaging and analysis

Drosophila Retina Airyscan 3D - Maximum intensity projection

Increase Your Productivity

• Save time on investigations into localization and interaction of proteins that require multiple fluorescent labels: You collect all these signals in one go
• Perform simultaneous spectral detection in a single scan with the highest number of descanned or non descanned channels – including GaAsP technology
• Take full advantage of large fields of view and the highest speed of any linear scanning confocal – up to 13 fps

The Airyscan Principle

A classic confocal microscope illuminates one spot on your sample to detect the emitted fluorescence signal. Out-of-focus emission light is rejected at a pinhole, the size of which determines how much of the airy pattern reaches the detector. You can increase the resolution by making the pinhole smaller, but signal-to-noise drops significantly since less valuable emission light is passing through. With Airyscan ZEISS introduces a new concept. Instead of throwing light away at the pinhole, a 32 channel area detector collects all light of an Airy pattern simultaneously. Each detector element functions as a single, very small pinhole. Knowing the beampath and the spatial distribution of each Airy pattern enables a very light efficient imaging: you can now use all of the photons that your objective collected.

It's up to you whether you use the additional information from your sample to get better signal-to-noise, resolution or speed. In the optional Virtual Pinhole Mode, you can decide even after the acquisition, which pinhole size best suits your application.

_{Chloroplasts of Elodea spec., 512 x 512 pixel at 10 frames per second with a pixel integration time of 0.36 microseconds}

The Airyscan Principle

_{LSM 880 Airyscan Beampath and Airyscan Principle}